RuralSymbiosis addresses a critical challenge in the BSR: the underutilisation of agricultural and forestry side-streams. Although biomass residues and by-products hold significant potential, small rural enterprises often lack the scale, logistics, and cooperation needed to valorise them. Traditional, centralised industrial symbiosis and innovation models do not fit the dispersed and seasonal realities of rural biomass, leaving valuable resources treated as low-value waste. The project’s main target groups—regional authorities, development agencies, research centres, and business support organisations (multiplier organisations)—struggle to bridge the gap between high-level bioeconomy policies and grassroots needs. They face weak cooperation networks, limited trust among stakeholders, and a lack of practical tools to connect SMEs with solution providers. RuralSymbiosis addresses these barriers by demonstrating practical symbiosis models through study visits, stakeholder workshops, open innovation events, and crucially, by building the capacity of these multiplier organisations. Its key deliverables include the Rural Symbiosis Toolbox—a set of actionable methods to foster cooperation, generate innovative valorisation solutions, and strengthen rural economies—and regional rural symbiosis roadmaps for each participating region, which will provide tailored guidance to embed project outcomes into long-term regional strategies, ensuring lasting impact beyond the project’s lifetime.